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Cooking Spanish

By: Kay Scarlett [Publisher]

Price: $18.00

Publisher: Sydney, NSW, Murdoch Books : 2005

Seller ID: 268125

ISBN-13: 9781740454742

Binding:Softcover

Condition: Very Good Condition

Spanish cuisine is wonderfully rich in seafood. Salmon, tuna, shrimp, clams, oysters, and scallops are all featured here in authentic recipes flavored with Spain's other national tastes-olive oil, wine, sea salt, almonds, sherry, and an abundance of vegetables and fruits. Cooking Spanish opens with an extensive chapter on "Tapas Traditions," and 250 color photographs show the mouth-watering results. Both practical and inspirational, Cooking Spanish will bring popular Spanish nibbles into 192 pages. Quantity Available: 1. Shipped Weight: 1-2 kilos. Category: Cookery, Food & Wine; Australia; Coo...
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Research and scholarship

Krysia Dziedzic is a Professor of Musculoskeletal Therapies and Director of the Impact Accelerator Unit, School of Medicine, Keele University, UK. An NIHR Senior Investigator, Krysia is also a Visiting Professor at the University of the West of England and a Fellow of the Chartered Society of Physiotherapy. She is a member of the NIHR School for Primary Care Research and the NIHR West Midlands Applied Health Research Collaboration. Krysia has led research and knowledge mobilisation in primary care for the uptake of NICE guidelines and quality standards for the care and management of osteoarthritis. She has a passion for patient and public involvement and engagement in research and implementation and is a member of the NIHR Race Equality Public Action Group (NIHR). Currently, Krysia is the Chair of the Implementation Subgroup for the Joint Effort Initiative (JEI) for Osteoarthritis as part of the Osteoarthritis Research Society International (OARSI), and convenor of the network JIGSAW-E (Joint Implementation of Guidelines for osteoarthritis in Western Europe).

Impact Accelerator Unit (IAU)

The IAU aims to maximise the benefits of world-leading health and care research, making an impact on the quality of life and care for patients and the public. The overarching objective is to have a positive and sustained impact on public health, health and social care, by supporting the timely movement of Keele's health research into practice, with a strong emphasis on primary care.

Abstract

One of the biggest challenges for in vivo gene therapy are vectors mediating highly selective gene transfer into a defined population of therapy-relevant cells. Here we present DARPin-targeted AAVs (DART-AAVs) displaying DARPins specific for human and murine CD8. Insertion of DARPins into the GH2/GH3 loop of the capsid protein 1 (VP1) of AAV2 and AAV6 resulted in high selectivity for CD8-positive T cells with unimpaired gene delivery activity. Remarkably, the capsid core structure was unaltered with protruding DARPins detectable. In complex primary cell mixtures, including donor blood or systemic injections into mice, the CD8-targeted AAVs were by far superior to unmodified AAV2 and AAV6 in terms of selectivity, target cell viability, and gene transfer rates. In vivo, up to 80% of activated CD8+ T cells were hit upon a single vector injection into conditioned humanized or immunocompetent mice. While gene transfer rates decreased significantly under non-activated conditions, genomic modification selectively in CD8+ T cells was still detectable upon Cre delivery into indicator mice. In both mouse models, selectivity for CD8+ T cells was close to absolute with exceptional detargeting from liver. The CD8-AAVs described here expand strategies for immunological research and in vivo gene therapy options.

Keywords: gene therapy, viral vectors, targeted AAV, AAV capsid engineering, targeted gene therapy, in vivo gene therapy, in vivo delivery, T cell targeting, receptor targeting, DART-AAVs, CD8 targeting, CD8-AAV, T cell activation

Graphical abstract

Demircan, Zinser and colleagues describe serotype 2 and 6 DART-AAVs targeted to mouse or human CD8. They demonstrate unaltered capsid core structures with protruding DARPins. In syngeneic and humanized mice, they find high in vivo selectivity for CD8+ T lymphocytes and identify a clinically translatable regimen to boost gene transfer.

Introduction

In the realm of genetic m